Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions

A growing interest has recently emerged in the use of nanomaterials medical applications. Nanomaterials, such as MXene, have unique properties due to their 2D ultra-thin structure, which is potentially useful cancer photothermal therapy. To be most effective, agents need internalized by cells. In this study, MXene was fabricated using chemical reactions and tested a agent on MDA-231 breast cells under static physiological conditions. Fluid shear stress (∼0.1 Dyn/cm 2 ) applied perfusion system mimic tumor microenvironment. The uptake analyzed fluid flow compared culture confocal microscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), transmission (TEM). Furthermore, viability assay used assess cell’s survival after exposing treated laser at different power densities durations. We showed that when incubated with cells, nanoparticles were successfully into resulting increased intracellular temperatures exposed NIR laser. Interestingly, dynamic alone did not result significant increase suggesting for surface modifications enhanced cellular stress.